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Nucleolus (RNA) probes

F22 [Catalog # P023]

■ Chemical structure & Information
F22 is a selective RNA probe over DNA. F22 was disclosed through in vitro DNA/RNA selectivity screening and live cell staining for nucleolus
■ Cell Image
Live Cell RNA Staining with Selected Dyes E36, E144, and F22 were tested at a 5 mM concentration. The picture of F22-stained 3T3 cells was obtained in a 1 mM dye concentration.
1000x magnification was utilized in the imaging. The scale bar represents 5 mm. Image brightness and contrast were slightly adjusted to improve picture quality. E36, E144 (green: FITC channel), and F22 (red: Cy3 channel) are shown.
■ Related probes: E36, F22
■ Known Property
RNA probe
■ Application
Selective fluorescent imaging of nuclear structure in live cells
■ Cell selectivity mechanism
RNA
■ Reference
1.
RNA-selective, live cell imaging probes for studying nuclear structure and function., Li, Q., Kim, Y. K., Namm, J., Kulkarni, A., Rosania, G., Ahn, Y. H., Chang, Y. T.* Chem. Biol. 2006, 13, 615-623.
2.
A protocol for preparing, characterizing and using three RNA-specific, live cell imaging probes: E36, E144 and F22 Li, Q.; Chang, Y. T.* Nat. Protoc. 2006, 1, 2922-2932.
3.
RNA buffers the phase separation behavior of prion-like RNA binding proteins, Maharana, S.; Wang, J.; Papadopoulos, D. K.; Richter, D.; Pozniakovsky, A.; Poser, I.; Bickle, M.; Rizk, S.; Guillén-Boixet, J.; Franzmann, T.; Jahnel, M.; Marrone, L.; Chang, Y. T.; Sterneckert, J.; Tomancak, P.; Hyman, A. A.; Alberti, S. Science 2018, 360, 918-921.
4.
RNA-Induced Conformational Switching and Clustering of G3BP Drive Stress Granule Assembly by Condensation, Guillén-Boixet, J.; Kopach, A.; Holehouse, A. S.; Wittmann, S.; Jahnel, M.; Schlüßler, R.; Kim, K.; Trussina, I. R. E. A.; Wang, J.; Mateju, D.; Poser, I.; Maharana, S.; Ruer-Gruß, M.; Richter, D.; Zhang, X.; Chang, Y. T.; Guck, J.; Honigmann, A.; Mahamid, J.;
Hyman, A. A.; Pappu, R. V.; Alberti, S.; Franzmann, T. M. Cell 2020, 181, 346-361.

■ ORDER
International
USD 350 + VAT (10%) + Fedex delivery charge
In Korea
KRW 450,000 + VAT (10%)
100 nmol (dried form: equivalent to 1 mM / 100 µL)

■ Storage
① Delivery: Room Temperature
② Dried compound: 4°C or -20°C
③ Compound solution: 4°C or -20°C

■ General Use Guide
•
More than 1/100 dilution of 10mM of DMSO stock solution is essential
•
For biomedical use to avoid DMSO concentration higher than 1%.
•
Working concentrations for specific applications should be determined by the investigator.
•
It is recommended to use up the buffer diluted solution within one day. The compound may be decomposed or
precipitated out from buffer solution.
■ Data sheet PDF file
F22 data sheet v0.2.pdf643.34KB
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